Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen

WANG Yuting; PENG Yuanyuan; LIU Xia; CHEN Xueqin; ZHAO Linjing

doi:10.12299/jsues.22-0054

Volume 37 Issue 2

Jun. 2023

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Article Navigation > Journal of Shanghai University of Engineering Science > 2023 > 37(2): 128-132

WANG Yuting, PENG Yuanyuan, LIU Xia, CHEN Xueqin, ZHAO Linjing. Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen[J]. Journal of Shanghai University of Engineering Science, 2023, 37(2): 128-132. doi: 10.12299/jsues.22-0054

Citation:

WANG Yuting, PENG Yuanyuan, LIU Xia, CHEN Xueqin, ZHAO Linjing. Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen[J]. Journal of Shanghai University of Engineering Science, 2023, 37(2): 128-132. doi: 10.12299/jsues.22-0054

Citation:

WANG Yuting, PENG Yuanyuan, LIU Xia, CHEN Xueqin, ZHAO Linjing. Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen[J]. Journal of Shanghai University of Engineering Science, 2023, 37(2): 128-132. doi: 10.12299/jsues.22-0054

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Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen

doi: 10.12299/jsues.22-0054

School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China

Received Date: 2022-03-13
Publish Date: 2023-06-20

Abstract

Abstract

The effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen were investigated. The polysaccharide of northern Ban-Lan-Gen was extracted by conventional reflux extraction method and ultrasonic assisted reflux extraction method. The total polysaccharide content was determined by phenol-sulfuric acid spectrophotometry. DPPH free radical and ABTS⁺ free radical scavenging assays were used to evaluate their antioxidant activity in vitro. The results show that the yield of polysaccharide extracted by ultrasonic assisted reflux extraction method was 40.07%, which was significantly higher than that by conventional reflux extraction method (13.42%). The phenol-sulfuric acid method has high precision, repeatability, stability and good recovery, which can be used to study the polysaccharide content of northern Ban-Lan-Gen prepared by different extraction methods. The polysaccharide content of ultrasonic assisted reflux extraction method was 22.3% higher than that of conventional reflux extraction method, and the scavenging effects of DPPH· and ABTS⁺· was significantly better than that of conventional reflux extraction method. The results provide a reference for the application of ultrasonic technology in the extraction of polysaccharide of northern Ban-Lan-Gen.
- northern Ban-Lan-Gen,
- polysaccharide,
- ultrasonic assisted extraction,
- antioxidant activity,
- ABTS⁺ free radical (ABTS⁺·),
- DPPH free radical (DPPH·)

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References(8)

References

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